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My research works
Bird’s-eye view of frog retina by SEM
Supra molecular organization of rod outer segments
Freeze-etched replicas of rod outer segments of photoreceptor cells
Localization of anti-opsin antibodies：Difference in fixation
Immuno-localization of anti-opsin
Morphogenesis of rod outer segments
Polarity of actin filaments that are found at the tip of the connecting cilium : S1 decoration
Electron micrographs of synaptic ribbons
Synaptic ribbons observed from various points of view at mesoscale
Anti-Caveolin 1 antibodies are found on the synaptic ribbon
Elastic and inelastic scattered electrons
Optical pass way in the electron microscope equipped with γ type energy filter
EELS imaging of Ca in cone synaptic pedicle
Ca localization determined by EELS imaging
Membrane and cytoplasmic cytoskeleton
Spatial distribution of membrane actin filaments
Live cell imaging of GFP-actin in NRK cells
Cytoplasmic surface of cell membrane covered with many actin filaments
Clathrin and actin filaments
Few membrane cytoskeletons are detected even in grazing thin section,
Tomography of membrane cytoskeleton
Actin filaments are classified into three types based on spatial distribution
AFM imaging of membrane cytoskeleton in water
AFM imaging of membrane cytoskeleton in water (2)
AFM imaging of clathrin coats in water
Whole cell observation
Cryo-electron micrograph of native cytoskeleton beneath the cell membrane
Cryo-electron micrograph of native cytoskeleton beneath the cell membrane (2)
Cryo-electron micrograph of native cytoskeleton beneath the cell membrane (3)
Spatial Specificity of Actin Cytoskeletons found by immuno-labeling
Protocol of immuno-freeze-etching
Actin filaments are identified by labeling with colloidal gold conjugated antibody
Rac 5nm gold / Sla 1 10nm gold
N WASP 10nm gold / IQGAP 1 5 nm gold
Myosin II appeared to be more abundant on stress fibers than the other type of actin filaments on the membrane.
Focal contact related proteins [Integrin b1]
Focal contact related proteins [Zyxin]
Immuno-gold labeling (IQCAP-1) in cryo-electron microscopy
Cryo-electron micrograph showing the dominant area of caveoli labeled with anti-IQGAP1 immunogold
Cytoplasmic and Peri-nuclear actin filaments
CLSM visualize the cytoskeleton mainly at a bottom plane.
Cytoplasmic cytoskeleton observed by extremely high voltage (1000KV) TEM.
High voltage electron microscopic image of unroofed pigment epithelial cells.
Myosin II localization in cytoplasmic cytoskeleton
High resolution SEM image of cytoplasmic cytoskeleton
Actin filaments in the periphery of nucleus
Many actin filaments are extending from the nuclear envelope
Actin filaments are associated intimately with intermediate filaments on the nuclear envelope.
Grazing section of the nucleus labeled with SI
Actin and intermediate filaments originating from nuclear pores (periphery)
Back scattered electron image showing vimentin filaments extending from nuclear pores
Anti-vimentin immuno-golds were found predominantly in periphery of the nucleus.
Intermediate filaments covering the outer nuclear envelope were identified as Vimentin.
Anti-Nesprin 1 antibodies are detected predominantly near nuclear pores.