Biochemistry 2

Masayoshi MAESHIMA Professor

Department: School of Agricultural Sciences / Graduate School of Bioagricultural Sciences

Class Time: 2013 Spring Monday
Recommended for: School of Agricultural Sciences
2nd year students

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Course Overview

All organisms take foods and use them to get elements for their own components, such as proteins, and energy through various metabolisms including glycolysis, citric acid cycle, and energy-yielding metabolism.

The lecture provides basic information on chemical characteristics of the enzyme reactions, the detailed reactions of the glycolysis and citric acid cycle, biochemical regulation, and their physiological significance. Also this course examines how cells transduce energy obtained from glucose to ATP production through oxidative phosphorylation.

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Course Aims

Through the lecture, students will be able to:

  • understand the chemical logic and biochemical reactions of sugar metabolism and oxidative phosphorylation
  • define physicochemical logic of bioenergetics
  • understand general properties of enzymes
  • describe and calculate the parameters of enzyme reactions


Metabolism, glycolysis, citric acid cycle, urea cycle, enzyme kinetics, oxidative phosphorylation, ATP synthesis


Lehninger Princeples of Biochemistry, Sixth edition (Fifth edition for Japanese translation)


The instructors will pass out handouts in class. Other reading materials will be introduced by the instructors

Related Classes

Biochemistry 1, Basic chemistry II

Course Schedule

Part1 Instructor: MAESHIMA
1 General introduction
2 Cell compartments and their biochemical roles
3 Biochemical methods to analyze proteins (introduction for genomes, subcellular fractionation, protein purification, and functional analysis)
4 Enzyme kinetics as an approach to understanding metabolism (Enzyme kinetics, Michaelis-Menten equation, and Enzyme inhibitors)
Part2 Instructor: HORIO
5 Glycolysis
6 Regulation of metabolic pathway
7 Pentose phosphate pathway of glucose oxidation
8 Citric acid cycle
9 Oxidation of fatty acid
10 Amino acid oxidation and urea cycle
Part3 Instructor: MAESHIMA
11 Enzyme inhibitors and kinetic tests
12 Coenzymes and cofactors
13 Bioenergetics: ATP and high-energy phosphate compounds
14 Oxidative phosphorylation
15 Energy efficiency of ATP synthesis


Attendance (20%), final exam (80%)

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Page last updated May 29, 2014

The class contents were most recently updated on the date indicated. Please be aware that there may be some changes between the most recent year and the current page.

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